Part:BBa_V1019

HB101

Taken from http://openwetware.org/wiki/E._coli_genotypes#HB101

F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-

Please note that different sources have different genotypes so treat this information with caution.

• From a GIBCO BRL list of competent cells.
• Hybrid of E. coli K12 and E. coli B
• Host for pBR322 and many plasmids
• Sigma lists the deletion Δ(gpt,proA). Check this.
• Promega does not list F-, mcrB, or mrr
• Streptomycin resistant

• References:
  • Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.
  • Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.
  • Lacks S and Greenberg JR (1977) J Mol Biol 114:153.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]